Evaluation of pork quality in two
different genotypes and potential
of vitamin E to improve pork quality

 

J.L. Hasty, E. van Heugten, and M.T. See

 

Summary

The effects of vitamin E supplementation (0, 75, 150, 300, and 600 mg/kg) for 6 weeks prior to slaughter on pork quality of two different genotypes was evaluated. Berkshire pigs had improved meat quality as measured by water holding capacity and ultimate pH compared to Hampshire pigs.  Vitamin E supplementation for 6 weeks at levels up to 600 mg/kg increased vitamin E content of loin muscle, but did not appear to improve water holding capacity or ultimate pH in either genotype.

 

Introduction

Pork quality is one of the big issues facing the pork industry today.  With differing preferences from the export and domestic market, providing the consumer with an appealing product has become even more difficult.  The elimination of Pale Soft and Exudative (PSE) pork from the market has become more of a challenge with the incorporation of leaner breeds into production to help satisfy the consumers demand for a healthier and leaner product.  Environment, genetics and nutrition all play a role in the occurrence of PSE pork.   Nutritional stragies such as feeding high levels of vitamin E have shown potential for improving pig performance early in the grower phase (Asghar et al, 1991) and possible improvement in pork quality.  Genetics also plays a significant role in the quality of pork that enters the market.  The NPPC Terminal Line Genetic Evaluation Program (1994), which evaluated various breeds in loin meat quality traits, showed Berkshire breeds to have superior traits and Hampshire breeds to produce low quality meat according to parameters measured.  The objectives of this research were to: 1) evaluate the potential water holding capacity  (WHC) and pH in pigs of two genotypes using the shot biopsy technique, and 2) to evaluate the effect of feeding high levels of vitamin E on pig performance, WHC, 24 hour drip loss, and pH. Our hypothesis was that vitamin E would improve pork quality within the two genotypes, with the greatest improvement in the Hampshire pigs.

 

Materials and Methods

A total of 240 pigs was used (120 Berkshire x PIC and 120 Hampshire x PIC) and allotted within genotype to one of 5 dietary treatments based on body weight and sex.   Pigs were housed 3 pigs per pen at the NCSU Swine Evaluation Station, Clayton, NC.  The diets (Table 1) were standard corn and soybean meal (0.83% lysine) diets with poultry fat as the fat source, stabilized with ethoxyquin.  A control diet was formulated containing the NRC requirement of 22 mg vitamin E/kg diet and treatment diets were supplemented with 75, 150, 300 and 600 mg vitamin E/kg from a-tocopheryl acetate.  Pigs were allowed ad libitum access to feed and water throughout the six-week feeding period. Body weights, and feed intake were taken on day 0, 21, and 42.  Biopsy samples were taken from the right loin on day 42 to determine meat quality characteristics before slaughter (Cheah et al., 1993).  Pigs were slaughtered at a commercial facility and slaughter data were taken at this time.  Lion initial pH (pHI; the pH measured 45 minutes after slaughter), and ultimate pH (pHU; the pH measured 24 hours after slaughter) were taken using a hand-pH meter.  WHC was measured 24 hours after slaughter using the filter paper method (Kauffman et al., 1986).  Loin samples were obtained and analysed for vitamin E concentration.

 

Table 1.  Diets

 

Ingredients

% of diet, as fed basis

Corn

77.8

SBM

17.05

Fat

2.5

Dical P          

1.5

Limestone

0.4

Salt

0.35

Vit/Min Premix

0.25

Lysine HCl

0.15

Vit E premix  

(+/-)

 

Calculated content

%

Crude Protein           

14.7

Crude Fat

6.02

Calcium         

0.57

Phosphorus  

0.61

 

Data were analyzed as a randomized complete block design with a factorial arrangement of treatments using the general linear models (GLM) procedures of SAS (1988) with factors being genotype, gender and vitamin E level.  Contrast comparisons were made to determine the effect of vitamin E supplementation versus control and to evaluate linear and quadratic effects of supplementation.

 

Results and Discussion

Pigs fed diets with increasing levels of vitamin E tended to have a higher (linear effect, P < 0.10) average daily feed intake (ADF), which was not affected by genotypes (Figure 1).   Average daily gain (ADG) was greater (P < 0.001) in Hampshire pigs compared to Berkshire pigs, with no effect of vitamin E (Figure 2).  Gain to feed ratio (G:F) was greater (P < 0.001) in Hampshire pigs compared to Berkshire pigs. Supplementation of vitamin E to the diet tended to decrease (P < 0.01) G:F (Figure 3). Increasing vitamin E in the diet increased the concentration of vitamin E (P < 0.001; linear effect, 2.02 to 5.92 mg/kg) in the loin for both genotypes, but there was no effect of genotype (Figure 4). 


 

Figures 1 and 2. Effect of vitamin E and genotype on Average Daily Feed Intake and Average Daily Gain.

 

 

Figures 3 and 4. Effect of vitamin E and genotype on Gain:Feed and day 0 pork chop vitamin E concentration.

 

Biopsy fluid recovery tended to be higher (P < 0.01) in the Hampshire pigs than in the Berkshire pigs, indicating that the muscle from Hampshire pigs was less capable of retaining water. Vitamin E supplementation had no significant effect on biopsy fluid recovery amounts and there was no interaction between genotype and vitamin E supplementation (Figure 5).  Biopsy fluid pH tended to be higher (P < 0.10) in the Hampshire pigs, but vitamin E had no significant effect (Figure 6).  Drip loss measured 24 hours after slaughter was greater in Hampshire pigs (Figure 7), which was in agreement with the results obtained using the biopsy procedure.   Ultimate pH (pHu), was reduced in Hampshire pigs (P < 0.001; Figure 8) compared to Berkshire pigs.  These results are consistent with the observations by NPPC (1994) indicating that Hampshire pigs have poorer meat quality than Berkshire pigs.  Supplementation to the diet of vitamin E did not affect drip loss or ultimate pH (Figures 7 and 8).

 


 

Figures 5 and 6. Effect of vitamin E and genotype on biopsy fluid recovery and biopsy fluid pH.

 

 

Figures 7 and 8. Effect of vitamin E and genotype on 24 hour drip loss and ultimate pH.

 

References:

Asghar,A., J. I. Gray, E. R. Miller, P.K. Ku, A. M. Booren, and D. H. Buckley. 1991b. 

Influence of supranutritional vitamin E supplementation in the feed on swine  

growth performance and deposition in different tissues. J. Sci. Food Agric. 57:19.

Cheah, L. S., A. M. Cheah, R. Lahucky, J. Mojto, and L. Kovac.  1993.  Prediction of

meat quality in live pig using stress-suceptible and stress-resistant animals.

Meat Science. 34:179-189.

Kauffman, R. G., G. Eikelenboom, P. G. van der Wal, G. Nerkus, and M. Zaar. 1986. 

The use of filter paper to estimate drip loss of porcine musculature.  Meat

Science.  18:191-200.

Morgan, J.B., G.C. Smith, J. Cannon, F. McKeith, and L. Heavner.  1994.  Pork

distribution channel audit report.  Pork Chain Quality Audit, Progress Report,

April 6, 1994. p. 30.

NPPC.  1995.  Genetic evaluation:  Terminal line program results.  National Pork

Producers Council publication, Des Moines, IA.